Standardization of Agrobacterium mediated genetic transformation in Indica rice cv BPT-5204
نویسندگان
چکیده
منابع مشابه
Optimization of Agrobacterium-Mediated Genetic Transformation in indica Rice
This experiment was conducted during the period from August 2004 to April 2005 in the Biotechnology & Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh. In this experiment, an efficient and reproducible protocol for the production of transgenic rice plants was developed by inoculating mature embryo of three indica ric...
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An efficient Agrobacterium-mediate transformation method was developed by employing different duration of sonication, Agrobacterium strains, type of inoculation medium and concentrations of acetosyringone in Arya cultivar of wheat. Immature embryos were used as an explant for inoculation with Agrobacterium tumefaciens harboring the recombinant pBI121 plasmid. Among the durations of sonication t...
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Agrobacterium tumefaciens strain EHA 105 carrying binary vector pCAMBIA 1301 was used for transformation in two economically important highly recalcitrant indica rice cultivars HKR-46 and HKR126. High concentrations of acetosyringone in the Agrobacterium culture and co-cultivation medium proved to be indispensable for successful transformation. Embryogenic scutellar calli were used for transfor...
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We report successful Agrobacterium-mediated transformation of a popular rice variety White Ponni, a non-basmati indica rice (Oryza sativa L.). Scutellumderived calluses of White Ponni were transformed with Agrobacterium strain LBA4404 (pSB1) harbouring the binary vector pMKU-RF2 with rice chi11 gene. Five independent transgenic White Ponni plants were ge nerated from hygromycin-resistant callus...
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Agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants. In this study, the vectors pMF-2A∗ containing a synthetic cry2A∗ gene driven by maize ubiquitin promoter and pCAMBIA1301 harboring hygromycin phosphotransferase gene (hpt) were introduced into Minghui86 (Oryza sativa L. ssp. indica), an elite indica restorer line. Two independent transfo...
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ژورنال
عنوان ژورنال: Annals of Plant Sciences
سال: 2018
ISSN: 2287-688X
DOI: 10.21746/aps.2018.7.2.9